Mass spectrometry of underivatized 15-hydroxyeicosatetraenoic acid and 15-hydroxyeicosapentaenoic acid

✍ Scribed by Karsten Fogh; Knud Kragballe; Elfinn Larsen; Helge Egsgaard; Vijai K. S. Shukla

• Book ID: 101757752
• Publisher: John Wiley and Sons
• Year: 1988
• Tongue: English
• Weight: 241 KB
• Volume: 17
• Category: Article
• ISSN: 1076-5174
• DOI: 10.1002/bms.1200170608

✦ Synopsis

A direct mass spectrometric method for 15-hydroxy metabolites of arachidonic acid and of eicosapentaenoic acid is described. 15-Hydroxyeicosanoids have the capacity to inhibit the formation of leukotrienes, potent mediators of inflammation formed by the 5-lipoxygenase pathway of arachidonic acid metabolism and may therefore have anti-inflammatory properties. 15-Hydroxyeicosatetraenoic acid (15-HETE) and 15-hydroxyeicosapentaenoic acid (15-HEPE) were synthesized using soybean lipoxygenase and arachidonic acid and eicosapentaenoic acid as sub- strates. These hydroxy fatty acids were then purified by reversed-phase high-performance liquid chromatography. This modified procedure provides rapid synthesis of multimilligram quantities of 15hydroxyeicosanoids for in vitro and in vivo studies. Electron impact mass spectra of 15-HETE and 15-HEPE could be obtained directly without deriva tiza tions.