A new strategy for the characterization of Coomassie Brilliant Blue stained SDS-PAGE separated proteins by UV-MALDI-MS is reported. The proteins are extracted directly from the polyacrylamide gel by treatment with an organic solvent mixture consisting of formic acid, acetonitrile, isopropanol and wa
β¦ LIBER β¦
Mass Spectrometry of Proteins Directly from Polyacrylamide Gels
β Scribed by Ogorzalek Loo, Rachel R.; Stevenson, Tracy I.; Mitchell, Charles; Loo, Joseph A.; Andrews, Philip C.
- Book ID
- 126271621
- Publisher
- American Chemical Society
- Year
- 1996
- Tongue
- English
- Weight
- 394 KB
- Volume
- 68
- Category
- Article
- ISSN
- 0003-2700
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