Mass Spectrometry of Proteins and Peptides Volume 146 || De Novo Peptide Sequencing by Nanoelectrospray Tandem Mass Spectrometry Using Triple Quadrupole and Quadrupole/Time-of-Flight Instruments
β Scribed by Chapman, John R.
- Book ID
- 118071534
- Publisher
- Humana Press
- Year
- 2000
- Weight
- 163 KB
- Category
- Article
- ISBN-13
- 9781592590452
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Protein microanalysis usually involves the sequencing of gel-separated proteins available in very small amounts. While mass spectrometry has become the method of choice for identifying proteins in databases, in almost all laboratories 'de novo' protein sequencing is still performed by Edman degradat
This paper describes experience with the commercially available LCQ quadrupole ion trap mass spectrometer applied to the o β -line analysis of peptides and proteins. The standard front end of the electrospray probe was replaced with a micromanipulator which, with the aid of a magnifying device, allow