Mass Spectrometry for the Clinical Laboratory

✍ Scribed by Hari Nair and William Clarke (Eds.)

• Publisher: Academic Press
• Year: 2016
• Tongue: English
• Leaves: 286
• Edition: 1st Edition
• Category: Library

✦ Synopsis

Mass Spectrometry for the Clinical Laboratory is an accessible guide to mass spectrometry and the development, validation, and implementation of the most common assays seen in clinical labs. It provides readers with practical examples for assay development, and experimental design for validation to meet CLIA requirements, appropriate interference testing, measuring, validation of ion suppression/matrix effects, and quality control. These tools offer guidance on what type of instrumentation is optimal for each assay, what options are available, and the pros and cons of each. Readers will find a full set of tools that are either directly related to the assay they want to adopt or for an analogous assay they could use as an example.

Written by expert users of the most common assays found in a clinical laboratory (clinical chemists, toxicologists, and clinical pathologists practicing mass spectrometry), the book lays out how experts in the field have chosen their mass spectrometers, purchased, installed, validated, and brought them on line for routine testing.

The early chapters of the book covers what the practitioners have learned from years of experience, the challenges they have faced, and their recommendations on how to build and validate assays to avoid problems. These chapters also include recommendations for maintaining continuity of quality in testing. The later parts of the book focuses on specific types of assays (therapeutic drugs, Vitamin D, hormones, etc.). Each chapter in this section has been written by an expert practitioner of an assay that is currently running in his or her clinical lab.

✦ Table of Contents

Content:
Front matter,Copyright,List of Contributors,PrefaceEntitled to full textChapter 1 - Mass spectrometry in the clinical laboratory: determining the need and avoiding pitfalls, Pages 1-15, W. Clarke
Chapter 2 - Application specific implementation of mass spectrometry platform in clinical laboratories, Pages 17-35, H. Nair
Chapter 3 - Sample preparation techniques for mass spectrometry in the clinical laboratory, Pages 37-62, J. Stone
Chapter 4 - Validation, quality control, and compliance practice for mass spectrometry assays in the clinical laboratory, Pages 63-76, D.F. Stickle, U. Garg
Chapter 5 - Best practices for routine operation of clinical mass spectrometry assays, Pages 77-107, B. Rappold
Chapter 6 - Toxicology: liquid chromatography mass spectrometry, Pages 109-130, K.L. Lynch
Chapter 7 - Toxicology: GCMS, Pages 131-163, P.B. Kyle
Chapter 8 - Therapeutic drug monitoring using mass spectrometry, Pages 165-179, P.J. Jannetto
Chapter 9 - Vitamin D metabolite quantitation by LC-MS/MS, Pages 181-204, H. Ketha, R.J. Singh
Chapter 10 - Steroid hormones, Pages 205-230, J.C. Cook-Botelho, L.M. Bachmann, D. French
Chapter 11 - Mass spectrometry in the clinical microbiology laboratory, Pages 231-245, I.W. Martin
Chapter 12 - High resolution accurate mass (HRAM) mass spectrometry, Pages 247-259, C.A. Crutchfield, W. Clarke
Chapter 13 - Evolving platforms for clinical mass spectrometry, Pages 261-276, J.Y. Yang, D.A. Herold
Index, Pages 277-288

✦ Subjects

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