Mass-Spectrometry-Linked Screening of Pr
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Joachim Jankowski; Nina Stephan; Martin Knobloch; Sven Fischer; Dominik Schmaltz
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Article
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2001
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Elsevier Science
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English
β 94 KB
A simple and rapid strategy is described to screen protein fractions for defined enzymatic activity. A protein fraction from a porcine kidney extract was immobilized by covalent coupling to activated affinity beads. The immobilized proteins were incubated with probes specific for different enzyme ac