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Mass spectrometry based metabolomics and enzymatic assays for functional genomics

✍ Scribed by Richard Baran; Wolfgang Reindl; Trent R Northen


Book ID
116747618
Publisher
Elsevier Science
Year
2009
Tongue
English
Weight
987 KB
Volume
12
Category
Article
ISSN
1369-5274

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A simple and rapid strategy is described to screen protein fractions for defined enzymatic activity. A protein fraction from a porcine kidney extract was immobilized by covalent coupling to activated affinity beads. The immobilized proteins were incubated with probes specific for different enzyme ac