Mass spectrometric structure determination of spider toxins: Arginine-containing acylpolyamines from venoms of Brazilian garden spider Nephilengys cruentata

✍ Scribed by Mario Sergio Palma; Yasuhiro Itagaki; Tsuyoshi Fujita; Miki Hisada; Hideo Naoki; Terumi Nakajima

• Book ID: 101212347
• Publisher: John Wiley and Sons
• Year: 1997
• Tongue: English
• Weight: 98 KB
• Volume: 5
• Category: Article
• ISSN: 1056-9014
• DOI: 10.1002/(sici)(1997)5:2<47::aid-nt1>3.0.co;2-x

✦ Synopsis

A new strategy to characterize glutaminergic blocker acylpolyamines stored in a spider venom with mass spectrometry is described. The crude spider venom extracts are amenable to direct MALDI mass spectrometry analysis which provides a rapid and accurate means of measuring the molecular weights of acylpolyamines without the isolation of individual samples. Compared with the previously developed m-column HPLC/MS method, this procedure provides more efficient detection and identification of complex venom constituents. Twenty-five acylpolyamines were detected from Brazilian garden spider Nephilengys cruentata crude venom extracts by both HPLC/MS and MALDI-mass spectrometry. These acylpolyamine structures were determined by high-energy collision induced dissociation MS/MS method. Most of the compounds were classified into the previously reported generalized structures types A to D, which were found from the venom of Nephilengys borbonica. The structures of four acylpolyamines (M 1 H) 1 , m/z 623, 646, 688, and 745, which were not contained in the venom of Nephilengys borbonicare were determined to have arginine at the polyamine chain terminal and were named respectively.