Mass spectrometric identification of ligands selected from combinatorial libraries using gel filtration
✍ Scribed by Yuriy M. Dunayevskiy; Jan-Ji Lai; Cheryl Quinn; Frank Talley; Paul Vouros
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 226 KB
- Volume
- 11
- Category
- Article
- ISSN
- 0951-4198
No coin nor oath required. For personal study only.
✦ Synopsis
There is a constant search for a successful analytical methodology to provide high throughput screening of combinatorial libraries against biological targets for identification of active ligands. Solid-phase screening assays offer faster isolation and identification of active analytes compared to the solution-based iterative methods. On the other hand, shift of combinatorial research to the creation of soluble non-peptide libraries, and limitations associated with the heterogeneous assays, creates a demand for a breakthrough technology for rapid and efficient screening of combinatorial libraries in solution. We demonstrated the efficient and rapid approach for selecting active ligands from a combinatorial mixture with subsequent identification of compounds by mass spectrometry. The procedure involves the use of a biological target molecule to physically isolate the active component in a mixture on a size exclusion medium. Then the ligands are identified using a combined liquid chromatography/capillary electrophoresis/mass spectrometry system. As a model system we used serum albumin and small molecules with different affinities to the protein.