Mass spectrometric identification of cyclic nucleotides released by the bacterium Corynebacterium murisepticum into the culture medium
✍ Scribed by Russell P. Newton; Eric E. Kingston; Alan Overton
- Publisher
- John Wiley and Sons
- Year
- 1998
- Tongue
- English
- Weight
- 188 KB
- Volume
- 12
- Category
- Article
- ISSN
- 0951-4198
No coin nor oath required. For personal study only.
✦ Synopsis
Controversial earlier evidence exists that
Corynebacterium murisepticum secretes the cyclic nucleotides cytidine 3',5'-cyclic monophosphate and inosine 3',5'-cyclic monophosphate. The nucleotide components of extracts of the bacterial cells and of the culture medium were purified and identified by chromatographic behaviour, UV absorbance/pH profile, base-to-phosphate ratio, acid hydrolysis and by fast-atom bombardment mass spectrometry combined with collisionally induced dissociation/mass-analysed ion kinetic energy spectrum scanning. This provides unambiguous identification of six cyclic nucleotides within the bacterial cells and four released into the growth medium. The significance of these findings and the potential future application of mass spectrometric methods in studies to elucidate the function of the released cyclic nucleotides is discussed.