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Mass spectrometric differentiation of huperzinine, N,N-dimethylhuperzine A and N-methylhuperzine B

✍ Scribed by Peifeng Hu; Michael L. Cross; S. Q. Yuan; T. T. Wei; Y. Q. Lu

Publisher
John Wiley and Sons
Year
1992
Tongue
English
Weight
488 KB
Volume
27
Category
Article
ISSN
1076-5174

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✦ Synopsis

The newly isolated

Lycopodium alkaloids huperzinine, N,N-dimethylhuperzine A and N-methylhupernne B were studied by high-resolution and tandem mass spectrometry. Differentiation of these subtly different isomers from each other and from the general class of Lycopodium alkaloids can be achieved by taking advantage of the structure-specific fragment ions formed upon electron ionization (EI) or collisional activation of these alkaloids. 8-Obscurine was identified as an impurity component in the N-methylhuperzine B sample and contributed to the EI mass spectrum. The previously published EI mass spectrum of N-methylhuperzine B is likely to be that of a mixture of N-methylhuperzine B and 8-obscurine. The presence of the impurity can now be readily recognized on the basis of two characteristic fragment ions of m/z 215 and 187.

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