Mass Spectrometric Determination of Methyl Farnesoate Profiles and Correlation with Ovarian Development in the Edible Crab,Cancer pagurus
✍ Scribed by Wainwright, G.; Prescott, M. C.; Rees, H. H.; Webster, S. G.
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 643 KB
- Volume
- 31
- Category
- Article
- ISSN
- 1076-5174
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✦ Synopsis
Evidence indicates that methyl farnesoate (MF), produced by the mandibular organs (MOs), is the crustacean equivalent of the juvenile hormones of insects. Gas chromatography-mass spectrometry with selected ion monitoring (GC-MSSIM) was used to measure the titers of MF in the MOs of the crab, Cancer pagurus. Comparison of the amounts of MF detected in groups of left and right MOs from crabs at different stages of ovarian development showed no statistically significant variation. The amounts of MF detected in MOs were in the range of 230 ng/MO in crabs with immature ovaries to 400 ng/MO in crabs with fully developed ovaries, Application of GC-MSSIM to the measurement of MF in hemolymph was unsuccessful, leading to the development of a high-performance liquid chromatography-MS-SIM (HPLC-MS-SIM) method for analysis of such titers. MF concentrations in hemolymph varied throughout ovarian development, exhibiting a peak at the beginning of secondary vitellogenesis (140 ng/ml), falling to basal levels thereafter.