Mass spectrometric and computational study of complex formation of nucleic acid bases with acrylamide as a surrogate for asparagine and glutamine residues
✍ Scribed by Igor K. Galetich; Vadim S. Shelkovsky; Marina V. Kosevich; Yury P. Blagoy; Nataly V. Shulyupina; Valery I. Poltev
- Publisher
- John Wiley and Sons
- Year
- 1998
- Tongue
- English
- Weight
- 57 KB
- Volume
- 12
- Category
- Article
- ISSN
- 0951-4198
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✦ Synopsis
In the context of the problem of nucleic acid-protein interactions, thermodynamic and geometrical parameters of complexes of methylated nucleic acid bases with acrylamide (as a model of the side chains of asparagine and glutamine) were investigated. Enthalpies of formation of nucleic acid base-acrylamide complexes, determined experimentally by means of temperature-dependent field ionization mass spectrometry, were in reasonable agreement with the interaction energies calculated theoretically using atom-atom potential functions. A stability order for base-acrylamide complexes was determined: m 9 Gua ! m 2 1,4 Cyt ! m 1 Cyt b m 9 Ade b m 3 1,4,4 Cyt b m 1 Ura b m 2 1,3 Thy. The geometries of the most energetically favourable complexes are determined and discussed.