Marking nitrocellulose membranes by peroxidase spotting
β Scribed by Ivano Eberini; Lina Puglisi
- Publisher
- John Wiley and Sons
- Year
- 1999
- Tongue
- English
- Weight
- 58 KB
- Volume
- 20
- Category
- Article
- ISSN
- 0173-0835
No coin nor oath required. For personal study only.
β¦ Synopsis
We describe how marking nitrocellulose membranes with horseradish peroxidase can be a useful and cheap method to obtain specific badges on zymograms, developed via enhanced chemiluminescence (ECL).
π SIMILAR VOLUMES
## Radial chromatography of serum proteins was investigated under various experimental conditions on nitrocellulose membranes. Development of about 15 ,ug/o.s ~1 samples on Sartorius 1x002 filters at pH 8.0-9.0, and with a salt concentration of up to 5 o/o NaCl at laboratory temperature (18-25") w
Various conditions were analyzed and optimized for the preparative elution of proteins from nitrocellulose membranes after transfer from sodium dodecyl sulfate (SDS)-polyacrylamide gels. The efficiency of elution was best using pyridine or acetonitrile elution solvents, intermediate for buffer conta