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Marker removal from actinomycetes genome using Flp recombinase

✍ Scribed by Marta Fedoryshyn; Lutz Petzke; Elisabeth Welle; Andreas Bechthold; Andriy Luzhetskyy


Book ID
116508054
Publisher
Elsevier Science
Year
2008
Tongue
English
Weight
234 KB
Volume
419
Category
Article
ISSN
0378-1119

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✍ Staub, J. M. ;Maliga, P. πŸ“‚ Article πŸ“… 1995 πŸ› Springer 🌐 English βš– 694 KB

We recently reported an 868-bp plastid DNA minicircle, NICE1, that formed during transformation in a transplastomic Nicotiana tabacum line. Shuttle plasmids containing NICE1 sequences were maintained extrachromosomally in plastids and shown to undergo recombination with NICE1 sequences on the plasti