MappingSBin relation toHLAandGL01using cells from first-cousin marriage offspring

✍ Scribed by Annemarie Termijtelen; P. Meera Khan; Stephen Shaw; Jon J. Rood

• Book ID: 104741106
• Publisher: Springer-Verlag
• Year: 1983
• Tongue: English
• Weight: 556 KB
• Volume: 18
• Category: Article
• ISSN: 0093-7711
• DOI: 10.1007/bf00364391

✦ Synopsis

A newly defined leukocyte antigen system, the secondary B-cell (SB) system, was shown to be linked to HLA. The SB marker has been investigated in lymphocyte donors presumed to be genetically homozygous for HLA-A through HLA-D/DR by virtue of descent from a first-cousin marriage and of phenotypic homozygosity for these HLA markers. Of 19 donors, 3 were found to be heterozygous for SB. Studies of the families of these three donors could not distinguish with certainty whether the heterozygosity resulted from SB/DR recombination or from "pseudohomozygosity" for HLA-A through -D/DR by inheritance of two genetically unrelated but similar haplotypes. However, our data favored the occurrence of SB/DR recombination with a meiotic distance perhaps as large as 3.3 cMorgan. Recombinations were identified which mapped SB between HLA-B and GL01. These studies demonstrate the usefulness of cells from first-cousin marriage offspring in mapping a polymorphic genetic system.