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Mapping the peptide and protein immune response in the larvae of the fleshfly Sarcophaga bullata

✍ Scribed by Alice Ciencialová; Tereza Neubauerová; Miloslav Šanda; Radek Šindelka; Josef Cvačka; Zdeněk Voburka; Miloš Buděšínský; Václav Kašička; Petra Sázelová; Veronika Šolínová; Martina Macková; Bohumír Koutek; Jiří Jiráček

Publisher
John Wiley and Sons
Year
2008
Tongue
English
Weight
401 KB
Volume
14
Category
Article
ISSN
1075-2617

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✦ Synopsis

Abstract

We chose the larvae of fleshfly Sarcophaga bullata to map the peptide and protein immune response. The hemolymph of the third‐instar larvae of S. bullata was used for isolation. The larvae were injected with bacterial suspension to induce an antimicrobial response. The hemolymph was separated into crude fractions, which were subdivided by RP‐HPLC, gel electrophoresis, and free‐flow electrophoresis. In several fractions, we determined significant antimicrobial activities against the pathogenic bacteria Escherichia coli, Staphylococcus aureus, or Pseudomonas aeruginosa. Among antimicrobially active compounds we identified dipeptide β‐alanyl‐ L‐tyrosine, protein transferrin, and two variants of peptide sapecin. We also partially characterized two novel antimicrobially active polypeptides; odorant‐binding protein 99b, and a peptide which remains unidentified. Copyright © 2007 European Peptide Society and John Wiley & Sons, Ltd.

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