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Mapping the Functional Topology of the Animal Fatty Acid Synthase by Mutant Complementation in Vitro †

✍ Scribed by Rangan, Vangipuram S.; Joshi, Anil K.; Smith, Stuart


Book ID
126801376
Publisher
American Chemical Society
Year
2001
Tongue
English
Weight
182 KB
Volume
40
Category
Article
ISSN
0006-2960

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In this work the Neurospora crassa al-3 gene function was determined. Geranylgeranyl pyrophosphate (GGPP) synthase activity was measured in al-2 FGSC 313 and al-3 RP100 FGSC 2082 mutant strains by in vitro synthesis methods. This experiment showed that al-3 RP100 mutant expresses a reduced GGPP synt