Mapping the entry of reactive oxygen metabolites into target erythrocytes during neutrophil-mediated antibody-dependent cellular cytotoxicity
✍ Scribed by Howard R. Petty; Bing Liang; Robert J. Maher
- Publisher
- John Wiley and Sons
- Year
- 1992
- Tongue
- English
- Weight
- 553 KB
- Volume
- 150
- Category
- Article
- ISSN
- 0021-9541
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✦ Synopsis
Transmitted Soret band optical microscopy has been used to image the entry and passage of reactive oxygen metabolites across target erythrocytes. Due to the rapid cytosolic diffusion of hemoglobin in comparison to video rates, it was necessary to use erythrocytes with relatively immobilized hemoglobin. To achieve this, erythrocytes from patients with sickle cell anemia were used. The movement of reactive oxygen metabolites across rabbit IgC-opsonized sickle cells was observed in real time. These observations indicate that reactive oxygen metabolites can enter and cross targets in an asymmetric fashion.