✦ LIBER ✦
Mapping small GTP-binding proteins on high-resolution two-dimensional gels by a combination of GTP binding and labeling with in situ periodateoxidized GTP
✍ Scribed by Dr. Lukas A. Huber; Marcus E. Peter
- Publisher
- John Wiley and Sons
- Year
- 1994
- Tongue
- English
- Weight
- 636 KB
- Volume
- 15
- Category
- Article
- ISSN
- 0173-0835
No coin nor oath required. For personal study only.
✦ Synopsis
Mapping small GTP-binding proteins on highresolution two-dimensional gels by a combination of GTP binding and labeling with in situ periodateoxidized GTP -We compared two approaches to identify and map small GTP-binding proteins in combination with high-resolution two-dimensional (2-D) gel electrophoresis. The first approach involved direct GTP ligand binding after a renaturing transfer onto nitrocellulose. In the second, affinity labeling with in situ periodate-oxidized GTP was used in permeabilized cells (Peter, M. E.,