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Mapping of the two mitochondrial antimycin a resistance loci in Saccharomyces cerevisiae

✍ Scribed by Michaelis, Georg ;Pratje, Elke

Publisher
Springer
Year
1977
Tongue
English
Weight
531 KB
Volume
156
Category
Article
ISSN
0026-8925

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✦ Synopsis

Retention or loss of the two new mitochondrial antimycin A resistance loci AI and AII has been analyzed in a large number of stable cytoplasmic petite mutants. Using these deletion mutants it was possible to localize the two antimycin A resistance loci in the OI--OII region of mitochondrial DNA. The genetic loci are mapped in the following order: OII--AI--AII--cobl--OI. The mapping relationship of mutants resistant to antimycin A or funiculosin to various cob mutants is described.

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Fifty eight mitochondrial mutants (p + mit- mutants), all deficient in cytochrome oxidase activity and previously assigned to the genetic region oxi3 on the mitochondrial DNA, were mapped by the method of "petite deletion mapping". This procedure resulted in the identification of at least twenty one