Mapping of the duplicated rabbit immunoglobulin x light chain locus
The rabbit has two isotypic forms of the immunoglobulin x light chain, K1 and K2, which probably arose by duplication. In the normal rabbit, only traces of K2 light chains are produced. However, K2 levels are elevated in allotype-suppressed rabbits and in the Basilea strain which does not produce K1 because of a K1 mFWA splice site mutation. Previous cloning and sequencing showed that each isotype has its own set of J, genes but it was not known whether the two isotypes utilize shared or separate sets of V, genes. In addition, although genetic linkage of allotypes associated with the K1 and K2 genes has been demonstrated, physical linkage had not been previously demonstrated by overlapping cosmid or phage clones. We used pulsed field and transverse alternating field electrophoresis to obtain megabase maps and to estimate the size of the duplication of the rabbit x tight chain locus.We found that the two C, genes are about 1 megabase apart. One explanation for the poor expression of K2, could be great physical distance from V, genes. However, we found that there are V,, J, and C,2 genes within a -105-kb fragment.Thus, physical distance of V, from C,2 may not be the basis for poor K2 expression.