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Mapping of the chicken N-CAM gene and a myosin heavy chain gene: Avian microchromosomes are not genetically inert reserves of DNA

✍ Scribed by Dominguez-Steglich, Marina ;Robbins, Jeffrey ;Schmid, Michael


Publisher
John Wiley and Sons
Year
1993
Tongue
English
Weight
611 KB
Volume
265
Category
Article
ISSN
0022-104X

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✦ Synopsis


Abstract

Physical mapping of the chicken neural cell adhesion molecule gene and of a chicken myosin fast‐white heavy chain gene via in situ hybridization has assigned both loci to different‐sized microchromosomes in this species. Two genomic cloned chicken DNA probes of 16 kb (λ cN6) and 15 kb (DCM 11), respectively, were biotin labeled for fluorescent detection of the genes. The implications derived from these results, together with further data just now beginning to accumulate regarding the genetic content of avian microchromosomes, confirm an unexpectedly active role for these minute chromosomes. © 1993 Wiley‐Liss, Inc.