Mapping of mutations affecting synthesis of exocellular enzymes in Bacillus subtilis

✍ Scribed by Steinmetz, Michel ;Kunst, Frank ;Dedonder, Raymond

• Book ID: 104726514
• Publisher: Springer
• Year: 1976
• Tongue: English
• Weight: 368 KB
• Volume: 148
• Category: Article
• ISSN: 0026-8925
• DOI: 10.1007/bf00332902

✦ Synopsis

The sacUh, amyB and pap mutations are identical with respect to their pleiotropic phenotype and their genetic location. Strains bearing these mutations overproduce several exocellular enzymes: alpha amylase, lavansucrase and proteases, they are poorly or not at all transformable and most of them are devoid of flagella. These mutations are tightly linked to the sacU- mutations by transformation and therefore lie between the hisA1 and gtaB290 markers. It is possible that the sacUh, amyB and pap mutations on one hand and the sacU- mutations on the other are two different classes of alterations of the same regulatory gene controlling the synthesis of some exocellular enzymes and several other cellular functions. Furthermore an amy- mutation, leading to the lack of alpha-amylase activity, was mapped between the lin2 and aroI906 markers which are not linked to the sacU locus.