Mapping of mutations affecting pyoverdine production in Pseudomonas aeruginosa
β Scribed by Dany Hohnadel; Dieter Haas; Jean-Marie Meyer
- Book ID
- 109313431
- Publisher
- John Wiley and Sons
- Year
- 1986
- Tongue
- English
- Weight
- 280 KB
- Volume
- 36
- Category
- Article
- ISSN
- 0378-1097
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## Abstract A novel application of the Smith/Birnstiel technique is presented for the analysis of intraspecies genomic diversity in small genomes. Rareβcutter total/__N__ frequentβcutter partial restriction digestions are separated in __N__ separate lanes by pulsedβfield gel electrophoresis, blotte
The nucleotide sequence required for a fully functional promoter and operator of the Pseudomonas aeruginosa argF gene (argFpo), the arginine-repressible gene for anabolic ornithine carbamoyltransferase, was defined within a 160 bp region. The streptomycin (Sm) resistance genes strAB of plasmid RSF10