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Mapping of ESTs derived from pre-implantation stage cattle embryos to allocate 16 new additions to the ordered comparative map of cattle and human

✍ Scribed by S. Mamo; K. Wimmers; M. Gilles; S. R. Kata; K. Schellander; S. Ponsuksili


Publisher
John Wiley and Sons
Year
2003
Tongue
English
Weight
61 KB
Volume
34
Category
Article
ISSN
0268-9146

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✦ Synopsis


Summary

Twenty expressed sequence tags (ESTs) derived from cDNA libraries of different developmental stages of embryos were mapped using a whole genome bovine hamster radiation hybrid panel. These include 14 markers representing genes, most of which have not so far been mapped in cattle, with another three being novel in both cattle and human. The markers were placed on specific chromosomes with high LOD scores and except two all localizations fit the current human and cattle comparative map. The assignment of these genes further enriches the cattle genome map and also contributes to the international effort of generating comparative maps.