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Mapping of 14 expressed sequence tags (ESTs) from porcine skeletal muscle by somatic cell hybrid analysis

✍ Scribed by R Davoli; D Bigi; L Fontanesi; P Zambonelli; M Yerle; C Zijlstra; A A Bosma; A Robic; V Russo

Publisher
John Wiley and Sons
Year
2000
Tongue
English
Weight
93 KB
Volume
31
Category
Article
ISSN
0268-9146

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✦ Synopsis

Chromosomal assignments are reported for fourteen porcine expressed sequence tags (ESTs) —CALM1, CRYAB, MYH7, MYL1, PDK4, PGAM2, PYGM, REV3L, RFC1, SLN, SPTBN1, SRM160, TPM1 and YWHAG. The ESTs were derived from our porcine skeletal muscle cDNA library. The ESTs sequences selected for mapping included the presence of the 3′‐untranslated region. The assignments were performed using two independent somatic cell hybrid panels providing the possibility of confirmation of the results obtained. The observed localizations are compared with the locations predicted from heterologous (human–pig, pig–human) chromosome painting data and knowledge of the map locations of the human homologues. These results add new information to the porcine genome transcript map.

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Isolation of expressed sequence tags of
Isolation of expressed sequence tags of skeletal muscle of neonatal healthy and splay leg piglets and mapping by somatic cell hybrid analysis
✍ S. Maak; S. Jaesert; K. Neumann; M. Yerle; G. Von Lengerken 📂 Article 📅 2001 🏛 John Wiley and Sons 🌐 English ⚖ 70 KB

We have isolated 14 differentially displayed and 10 further expressed sequence tags (ESTs) from Musculus biceps femoris of newborn healthy and splay leg piglets. By comparison with EMBL/GenBank data we could identify nine porcine homologues to human genes (TATA box binding protein associated factor