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Mapping cyclic AMP binding sites on type I and type II cyclic AMP-dependent protein kinases using 2-substituted derivatives of cyclic AMP

✍ Scribed by Terry S. Yagura; Caroline C. Sigman; Pricilla A. Sturm; Elmer J. Reist; Howard L. Johnson; Jon P. Miller


Book ID
118314402
Publisher
Elsevier Science
Year
1980
Tongue
English
Weight
396 KB
Volume
92
Category
Article
ISSN
0006-291X

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✍ Carlos J. Ciudad; Jordi Vila; M.Angels Mor; Joan J. Guinovart πŸ“‚ Article πŸ“… 1987 πŸ› Springer 🌐 English βš– 567 KB

Extracts obtained from rat hepatocytes incubated with saline, glucagon or insulin were electrophoresed on polyacrylamide gels and then assayed for cyclic (3H)AMP binding capacity. Analysis of the binding patterns demonstrated that glucagon dissociated a holoenzyme of cyclic AMP-dependent protein kin