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Mapping a Protein-Binding Site on Straightened DNA by Atomic Force Microscopy

โœ Scribed by Hiroki Yokota; Deborah A. Nickerson; Barbara J. Trask; Ger van den Engh; Martin Hirst; Ivan Sadowski; Ruedi Aebersold


Publisher
Elsevier Science
Year
1998
Tongue
English
Weight
257 KB
Volume
264
Category
Article
ISSN
0003-2697

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Atomic Force Microscopy-Based Detection
โœ Hui Bin Sun; Gerald N. Smith Jr.; Karen A. Hasty; Hiroki Yokota ๐Ÿ“‚ Article ๐Ÿ“… 2000 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 222 KB

Type II tropocollagen molecules were reacted with matrix metalloproteinase 8 (MMP-8) and the binding sites as well as the cleavage site of MMP-8 were detected on individual molecules using atomic force microscopy (AFM). Approximately 300-nm-long coiledcoil tropocollagen molecules were straightened a