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Mapping 2′-O-Methyl Groups in Ribosomal RNA

✍ Scribed by B.Edward H. Maden


Book ID
111693627
Publisher
Elsevier Science
Year
2001
Tongue
English
Weight
74 KB
Volume
25
Category
Article
ISSN
1046-2023

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Determination of 2′-O-methyl groups in 3
✍ I. Klein; J. Klootwijk 📂 Article 📅 1976 🏛 Elsevier Science 🌐 English ⚖ 742 KB

A two-dimensional method for the detection of ribose methyl groups in 32P-labeled RNAs is described which is suitable for both qualitative and quantitative purposes. The method involves fractionation of an alkaline hydrolysate of the RNA by two-dimensional electrophoresis of the digest on DEAEcellul