Strains carrying a dnaA temperature sensitive (t.s.) mutation and a Mu-1 prophage inserted within different genes near the origin of replication have been constructed. For each strain, integratively suppressed Hfrs, named G and D in which the ori region was replicated clockwise and counterclockwise
Map position of the replication terminus on the Escherichia coli chromosome
โ Scribed by Louarn, Jacqueline ;Patte, Josette ;Louarn, Jean-Michel
- Publisher
- Springer
- Year
- 1979
- Tongue
- English
- Weight
- 466 KB
- Volume
- 172
- Category
- Article
- ISSN
- 0026-8925
No coin nor oath required. For personal study only.
โฆ Synopsis
The directions of replication of several prophages integrated with a known orientation in the vicinity of the terminus (tre) of chromosome replication (trp::Mu, min 27; lambda rev integrated within rac, min 31, man::Mu, min 35), have been established by determining the molecular polarity of Okazaki pieces specific to these prophages. The results obtained strongly suggest that the site tre is located between rac and man, an otherwise genetically silent region.
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