Manganese inhibition of 3-acetyldeoxynivalenol biosynthesis inFusarium graminearumR 2118

✍ Scribed by Amit B. Vasavada; Dennis P. H. Hsieh

• Book ID: 104650883
• Publisher: Springer
• Year: 1990
• Tongue: English
• Weight: 565 KB
• Volume: 33
• Category: Article
• ISSN: 1432-0614
• DOI: 10.1007/bf00164532

✦ Synopsis

Biosynthesis of 3-acetyldeoxynivalenol (3-ADN) by Fusarium 9raminearurn R 2118 was inhibited in the presence of manganese (Mn ÷2) at 10 -3 to 50 m M in production medium. In this concentration range, toxin production was reduced by as much as 92%, while mycelial dry weight increased by as much as 50%. A rapid decline in p H accompanied the increased mycelial growth and intracellular accumulation of a yellow pigment. This inhibitory effect of Mn ÷2 on 3-ADN production was reversed by the presence of sodium citrate, sodium pyruvate, sodium acetate, or the chelating agent ethylenediaminetetraacetate (EDTA) in the medium but not by the presence of other organic acids of the tricarboxylic acid cycle or the protein synthesis inhibitors, chloramphenicol and cycloheximide. Evidence indicates that citric acid was playing a crucial role in 3-ADN biosynthesis and that Mn +2 was acting on the isocitrate dehydrogenase and acetyl coenzyme-A carboxylase sites to inhibit the toxin biosynthesis.