Malignant transformation of hamster embryo cells in tissue culture by 7,10-dimethyl benzo(c)acridine

✍ Scribed by P. Markovits; J. Coppey; D. Papadopoulo; A. Mazabraud; M. Hubert-Habart

• Publisher: John Wiley and Sons
• Year: 1974
• Tongue: French
• Weight: 761 KB
• Volume: 14
• Category: Article
• ISSN: 0020-7136
• DOI: 10.1002/ijc.2910140210

✦ Synopsis

Abstract

During the 4th passage of hamster embryo cells originally treated with 7,10‐dimethyl benzo (c)acridine, characteristic colonies with transformed cells appeared. This compound is carcinogenic in mice, but its effects on cells in culture have not yet been studied. Each cell line derived from this subculture produces sarcomas in new‐born hamsters. An inoculum of 10,000 cells per animal produced visible tumors in an average time of 67 days. If the number of transplanted cells was increased, the tumors appeared sooner. These experiments, as well as others carried out in our laboratory using other carcinogens, demonstrate that hamster embryo cells cultivated in vitro can be successfully used for transformation studies.