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MALDI-TOF mass spectrometry of a combinatorial peptide library: effect of matrix composition on signal suppression

✍ Scribed by Gitta Schlosser; Gabriella Pocsfalvi; Emőke Huszár; Antonio Malorni; Ferenc Hudecz

Book ID
102380669
Publisher
John Wiley and Sons
Year
2005
Tongue
English
Weight
207 KB
Volume
40
Category
Article
ISSN
1076-5174

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✦ Synopsis

Abstract

The effect of matrix composition on signal suppression caused by a dominant compound under MALDI ionization was studied using the combinatorial TQTXT pentapeptide library as a model system. The peptide library is composed of 19 components with all proteinogenic amino acids except cysteine in position X. From these compounds, only the Arg peptide (TQTRT) was detected with sufficient intensity in the MALDI‐TOF mass spectrum under typical MALDI conditions (CCA matrix). The analysis of a set of compounds utilized as different matrix components, additives and a cationizing agent revealed that the composition of the matrix is a critical point in signal suppression. Highly improved ion yields were achieved by using a CCA/DHB mixture as a matrix. The addition of K^+^ as a cationizing agent to the CCA matrix resulted in MALDI‐TOF mass spectra with relative ion intensities very similar to those obtained by electrospray ionization. Copyright © 2005 John Wiley & Sons, Ltd.

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