Malate synthase messenger RNA in relation to enzyme derepression inEuglena gracilis

✍ Scribed by Elaine Woodcock; Michael J. Merrett

• Publisher: Springer
• Year: 1980
• Tongue: English
• Weight: 557 KB
• Volume: 124
• Category: Article
• ISSN: 0302-8933
• DOI: 10.1007/bf00407025

✦ Synopsis

The production of poly (A) + RNA was determined over the cell-cycle in Euglena cultures division-synchronized by 14 h light periods alternating with 10 h dark periods. The amount ofpoly (A) + RNA per culture aliquot was constant from the beginning until near the end of the light phase of the cell-cycle when it increased in a sharp burst after the time of DNA replication. Poly (A) + RNA from all stages of the cellcycle promoted protein synthesis with equal efficiency in a mRNA-dependent reticulocyte lysate translation system. Malate synthase mRNA was detected using malate synthase antibody to precipitate nascent malate synthase protein in the translation system, binding the lgG-antigen complex to Staphylococcus aureus, concentrating, eluting the IgG-antigen complex and analysing the products by sodium dodecyl sulfatepolyacrylamide gel electrophoresis. Malate synthase mRNA was absent from phototrophic cells and was only produced in darkened cultures following the addition of acetate.