MAL64cis a global regulator of α-glucoside fermentation: identification of a new gene involved in melezitose fermentation

✍ Scribed by Edward L. Perkins; Richard B. Needleman

• Publisher: Springer-Verlag
• Year: 1988
• Tongue: English
• Weight: 694 KB
• Volume: 13
• Category: Article
• ISSN: 0172-8083
• DOI: 10.1007/bf00365657

✦ Synopsis

Maltase constitutive mutants at the MAL6 locus have been mapped to the newly identified regulatory gene MAL64 e. We show here that MAL64 c has in addition pleiotropic effects on sugar fermentation: MAL64 c strains constitutively synthesize an a-methylglucosidase and can complement a new gene, MTP1, for the fermentation of melezitose and a-methylglucoside. MTP1, maps near MALl, and either encodes a permease which transports melezitose, a-methylglucoside, and maltose or regulates the activity of such a permease. This work shows that MAL64 c, a transacting regulatory gene, is a global regulatory gene affecting several different pathways of a-glucoside metabolism.