Making ends meet in genetic analysis using padlock probes

✍ Scribed by Mats Nilsson; Johan Banér; Maritha Mendel-Hartvig; Fredrik Dahl; Dan-Oscar Antson; Mats Gullberg; Ulf Landegren

• Book ID: 102258606
• Publisher: John Wiley and Sons
• Year: 2002
• Tongue: English
• Weight: 321 KB
• Volume: 19
• Category: Article
• ISSN: 1059-7794
• DOI: 10.1002/humu.10073

✦ Synopsis

For the Mutation Detection 2001 Special Issue

Padlock probes are molecular tools that combine highly specific target sequence recognition with the potential for multiplexed analysis of large sets of target DNA or RNA sequences. In this brief review, we exemplify the ability of these probes to distinguish single-nucleotide target sequence variants. We further discuss means to detect the location of target sequences in situ, and to amplify reacted padlock probes via rolling-circle replication, as well as to sort reaction products on tagarrays. We argue that the probes have the potential to render high-throughput genetic analyses precise and affordable. Hum Mutat 19:410415, 2002.