Major Urinary Protein as a Negative Tumor Marker in Mouse Hepatocarcinogenesis

✍ Scribed by Tommaso A. Dragani; Giacomo Manenti; Marina R. M. Sacchi; Bruno M. Colombo; Giuseppe Della Porta

• Book ID: 102945348
• Publisher: John Wiley and Sons
• Year: 1989
• Tongue: English
• Weight: 564 KB
• Volume: 2
• Category: Article
• ISSN: 0899-1987
• DOI: 10.1002/mc.2940020610

✦ Synopsis

A cDNA clone isolated by differential screening of a mouse liver cDNA library using RNA probes from normal adult liver and from hepatocellular tumors showed, on northern blot analysis, a single transcript of 1 kb present at high levels in normal adult mouse liver but at 15-t o 100-fold reduced levels in spontaneous and pdimethylaminoazobenzene-or urethan-induced liver tumors. The transcript, which was absent in two-wkold mouse liver, is coded by a developmentally regulated gene. Nucleotide sequence analysis demonstrated that the isolated clone encoded a major urinary protein (MUP). lmmunocytochemistry analysis, using a goat antibody t o MUP on histologic liver sections containing diethylnitrosamine-induced liver nodules, showed that the decrease in MUP levels also occurred in microscopic nodules. Therefore, MUP decrease represents an early event in the development of mouse liver tumors, and it may be used as a tumor marker in mouse hepatocarcinogenesis.