✦ LIBER ✦

Major proteins of the outer cell envelope membrane of Escherichia coli K12: Multiple species of protein I differ in primary structure

✍ Scribed by Gamon, Konrad ;Chen, Robert ;Henning, Ulf

Publisher: Springer
Year: 1978
Tongue: English
Weight: 552 KB
Volume: 166
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00285921

No coin nor oath required. For personal study only.

✦ Synopsis

Protein I, one of the major outer membrane proteins of E. coli in most K12 strains is represented by two very similar polypeptides Ia and Ib. Sequential mutations (involving selections for phage resistance) can lead to loss of proteins Ia and Ib. Among "revertants" of such Ia-Ib- mutants clones exist that instead of Ia or Ib produce a third species of protein I, polypeptide Ic. Ichihara and Mizushima [J. Biochem. 83, 1095--1100 (1978)] have shown that proteins Ia and Ib exhibit differences in primary structure. Here evidence is presented indicating that protein Ic also is not identical in primary structure with Ia or Ib. Thus, 3 very similar structural genes appear to exist for the protein I species known to date, and that for Ic normally is silent. Introduction of a functional Ic locus into a Ia+ Ib+ strain caused expression of all three proteins with a reduced rate of synthesis of protein Ia.

📜 SIMILAR VOLUMES

Major proteins of the outer cell envelop

Major proteins of the outer cell envelope membrane ofEscherichia coli K-12: Multiple species of protein I

✍ Henning, Ulf ;Schmidmayr, Waltraud ;Hindennach, Ingrid 📂 Article 📅 1977 🏛 Springer 🌐 English ⚖ 519 KB

Lethal mutations in the structural gene

Lethal mutations in the structural gene of an outer membrane protein (OmpA) of Escherichia coli K12

✍ Freudl, Roland ;Braun, Gabi ;Hindennach, Ingrid ;Henning, Ulf 📂 Article 📅 1985 🏛 Springer 🌐 English ⚖ 786 KB

The gene ompA encodes a major outer membrane protein of Escherichia coli. Localized mutagenesis of the part of the gene corresponding to the 21-residue signal sequence and the first 45 residues of the protein resulted in alterations which caused cell lysis when expressed. DNA sequence analyses revea

Gene encoding a hybrid OmpF-PhoE pore pr

Gene encoding a hybrid OmpF-PhoE pore protein in the outer membrane of Escherichia coli K12

✍ Tommassen, Jan ;Pugsley, Anthony P. ;Korteland, Jaap ;Verbakel, John ;Lugtenberg 📂 Article 📅 1984 🏛 Springer 🌐 English ⚖ 685 KB

To study the structure-function relationship of outer membrane pore proteins of E. coli K12, a hybrid gene was constructed in which the DNA encoding amino acid residues 2-73 of the mature PhoE protein is replaced by the homologous part of the related ompF gene. The product of this gene is incorporat

The role of the carboxy-terminal membran

The role of the carboxy-terminal membrane-spanning fragment in the biogenesis of Escherichia coli K12 outer membrane protein PhoE

✍ Bosch, Dirk ;Scholten, Monica ;Verhagen, Cora ;Tommassen, Jan 📂 Article 📅 1989 🏛 Springer 🌐 English ⚖ 663 KB

Sequence of the regulatory region of omp

Sequence of the regulatory region of ompT, the gene specifying major outer membrane protein a (3b) of Escherichia coli K-12: Implications for regulation and processing

✍ Gordon, Gerald ;Gayda, Randall C. ;Markovitz, Alvin 📂 Article 📅 1984 🏛 Springer 🌐 English ⚖ 823 KB

The DNA of the promoter region of omp T, including the putative start for the pro-Omp T protein (pro-protein a), has been sequenced. Previous studies showed that trypsin inhibitors prevent the processing of pro-Omp T to Omp T protein which led to the prediction that the processing site would be a ly