Major Improvement of Lectin-Based Assays through Choice of Cation and Optimization of Cation Concentration
✍ Scribed by Mohammad M. Farajollahi; David B. Cook; Colin H. Self
- Publisher
- Elsevier Science
- Year
- 1998
- Tongue
- English
- Weight
- 76 KB
- Volume
- 261
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
✦ Synopsis
significant shrinkage for over a month. Storage at Ϫ20°C for over a month caused significant shrinkage without cracking (data not shown). Wet gel autoradiography in these bags helps reduce exposure to radioisotopes and avoids possible contamination of gel dryers in addition to saving time and effort. It also enables one to electrophoretically transfer fixed and stained gels if necessary (7). Of course, dried gels could be rehydrated and used. However, we have found that remnants of the filter paper (used for drying) remain stuck to the gel after rehydration, which have to be painstakingly removed to prevent the gel from tearing.
Storage of gels in polyethylene bags thus enables one to compare other gels for the reproducibility of protein spots and also to cut out protein spots when sufficient number of gels have accumulated. Stained and fixed gels stored in this manner could be used for electrophoretic transfer of proteins to nitrocellulose membrane (7), if necessary. It also provides an avenue for wet gel autoradiography thus removing the possibility of contaminating gel dryers. It also makes it possible to cut out bands for counting experiments or for other analysis.