Major histocompatibility complex class ii gene associations with anti–u1 small nuclear ribonucleoprotein antibody relationship to immunoreactivity with individual constituent proteins
✍ Scribed by Masataka Kuwana; Yutaka Okano; Junichi Kaburaki; Kimiyoshi Tsuji; Hidetoshi Inoko
- Publisher
- John Wiley and Sons
- Year
- 1995
- Tongue
- English
- Weight
- 1020 KB
- Volume
- 38
- Category
- Article
- ISSN
- 0004-3591
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✦ Synopsis
Relationship t o Immunoreactivity with Individual Constituent Proteins
MASATAKA KUWANA, Y UTAKA OKANO, JUNICHI KABURAKI, KIMIYOSHI TSUJI, and HIDETOSHI INOKO Objective. To better define immunogenetic associations with the anti-U1 ribonucleoprotein (U1 RNP) autoantibody response.
Methods. HLA class I1 alleles were determined by genotyping in 49 Japanese rheumatic disease patients with anti-U1 RNP antibody and 43 race-matched healthy controls. Immunoreactivities to U1 RNP constituent proteins (70K, A, B/IB', and C) were detected by immunoblots using purified HeLa cell Sm antigen, and antibody titer was determined by passive hemagglutination assay.
Results. DQB1*030:1 was significantly more frequent in anti-Ul RNP-positive patients than in controls (43% versus 14%; odds ratio [OR] = 4.6, corrected P = 0.03). All anti-U1 RNP-positive patients had either a DQBl"0601, *0602, *03011, *0302, or "0303 allele, which share tyrosine at potsition 30, and the amino acid sequence Thr, Arg, Ala, Glu, Leu, Asp, and Thr at positions 71-77 in the DQBl pl domain. In contrast, one of these alleles was found in 81% of the controls