Magnitudes and Orientations of the 15N Chemical Shift Tensor of [1-15N]-2′-Deoxyguanosine Determined on a Polycrystalline Sample by Two-Dimensional Solid-State NMR Spectroscopy

✍ Scribed by Gary A. Lorigan; Ronald McNamara; Roger A. Jones; Stanley J. Opella

• Publisher: Elsevier Science
• Year: 1999
• Tongue: English
• Weight: 72 KB
• Volume: 140
• Category: Article
• ISSN: 1090-7807
• DOI: 10.1006/jmre.1999.1822

✦ Synopsis

The magnitudes and orientations of the 15 N chemical shift tensor of [1-15 N]-2-deoxyguanosine were determined from a polycrystalline sample using the two-dimensional PISEMA experiment. The magnitudes of the principal values of the 15 N chemical shift tensor of the N1 nitrogen of [1-15 N]-2-deoxyguanosine were found to be 11 ‫؍‬ 54 ppm, 22 ‫؍‬ 148 ppm, and 33 ‫؍‬ 201 ppm with respect to ( 15 NH 4 ) 2 SO 4 in aqueous solution. Comparisons of experimental and simulated two-dimensional powder pattern spectra show that 33N is approximately collinear with the N-H bond. The tensor orientation of 33N for N1 of [1-15 N]-2-deoxyguanosine is similar to the values obtained for the side chain residues of 15 N ⑀1 -tryptophan and 15 N -histidine even though the magnitudes differ significantly.