β Scribed by D. I. Roseeuw; C. L. Marcelo; J. J. Voorhees
No coin nor oath required. For personal study only.
The polyamines, putrescine, spermidine and spermine, as well as ornithine decarboxylase (ODC), the rate-limiting enzyme ofpolyamine biosynthesis, are increased in the blood, urine and skin of psoriasis patients. A study of the association between stimulated epidermal cell proliferation and induction of ODC activity was done using both an intact, living cell ODC assay and the routinely used homogenized cell supernate assay. 10-6 to 10-8 mol/1 of the tumor promoter, tetradecanoyl phorbol acetate (TPA), I0-3m01/1 8-BrcAMP, 10 -6 tool/1 cholera toxin and 10-3mol/1 MIX, which are epidermal cell mitogens, were used to stimulate basal cells in short-term suspension cultures, freshly plated monolayers, or up to 8-day-old cultures. The results showed that the ODC enzyme must be assayed in supernates from disrupted epidermal cells in order for significant ODC induction by hyperplastic agents to be observed.
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## Abstract Nerve growth factor (NGF), epidermal growth factor (EGF), adenosine 3β²,5β²βcyclic monophosphate (cAMP), and cholera toxin all increase the specific activity of ornithine decarboxylase (ODC) in the PC12 nerveβlike cell line. A phosphodiesterase inhibitor augments the effect of NGF on the