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Magnitude of a finite equilibration effect in analytical gel chromatography

✍ Scribed by George H. Weiss; Gary K. Ackers

Publisher: Wiley (John Wiley & Sons)
Year: 1972
Tongue: English
Weight: 347 KB
Volume: 11
Category: Article
ISSN: 0006-3525
DOI: 10.1002/bip.1972.360111012

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✦ Synopsis

Abstract

Previous analyses of solute behavior in analytical gel chromatography^1,2^ have allowed for a finite equilibration time between stationary and mobile phases in estimating the magnitude of axial dispersion coefficients, but have ignored these effects in formulating the continuity equations for solute transport. We develop a more accurate theory including the equilibration effect, and show experimentally that it can be disregarded after approximately two minutes from the start of a small‐zone experiment.

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