✦ LIBER ✦
Magnetization Transfer via Residual Dipolar Couplings: Application to Proton–Proton Correlations in Partially Aligned Proteins
✍ Scribed by Maurizio Pellecchia; Craig W Vander Kooi; Kai Keliikuli; Erik R.P Zuiderweg
- Publisher
- Elsevier Science
- Year
- 2000
- Tongue
- English
- Weight
- 97 KB
- Volume
- 143
- Category
- Article
- ISSN
- 1090-7807
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✦ Synopsis
A novel three-dimensional NMR experiment is reported that allows the observation of correlations between amide and other protons via residual dipolar couplings in partially oriented proteins. The experiment is designed to permit quantitative measurement of the magnitude of proton-proton residual dipolar couplings in larger molecules and at higher degree of alignments. The observed couplings contain data valuable for protein resonance assignment, local protein structure refinement, and determination of low-resolution protein folds.