Magnetization transfer in cartilage and its constituent macromolecules

✍ Scribed by Martha L. Gray; Deborah Burstein; Leann M. Lesperance; Lee Gehrke

• Publisher: John Wiley and Sons
• Year: 1995
• Tongue: English
• Weight: 681 KB
• Volume: 34
• Category: Article
• ISSN: 0740-3194
• DOI: 10.1002/mrm.1910340307

✦ Synopsis

Abstract

The goal of this work was to investigate magnetization transfer (MT) in cartilage by measuring water proton signals M~s~/M~o~, as an indicator of MT, in (i) single‐component systems of the tissue's constituent macromolecules and (ii) intact cartilage under control conditions and after two pathomimetic interventions. M~s~/M~o~ was quantified with a 12‐μT saturation pulse applied 6 kHz off resonance. Both glycosaminoglycans (GAG) and collagen exhibited concentration dependent effects on M~s~/M~o~, being approximately linear for GAG solutions (M~s~/M~o~ = ‐0.0137[% GAG] + 1.02] and exponential for collagen suspensions (M~s~/M~o~ = 0.80 x exp[‐(%collagen)/6.66] + 0.20); the direct saturation of water could not account for the measured M~s~/M~o~. Although the effect of collagen on M~s~/M~o~ is much stronger than for a corresponding concentration of GAG, M~s~/M~o~ is not very sensitive to changes in collagen concentration in the physiological range. Tissue degradation with 25 mg/ml trypsin led to an increase in__M__~s~/M~o~ from the baseline value of 0.2 (final/initial values = 1.15 ± 0.13, n = 11, P < 0.001). In contrast, a 10‐day treatment of cartilage with 100 ng/ml of interleukin‐1β (1L‐1β) caused a 19% decrease in M~s~/M~o~ (final/initial values = 0.81 ± 0.08, n = 3, P = 0.085). The changes in hydration and macromolecular content for the two treatments were comparable, suggesting that M~s~/M~o~ is sensitive to macromolecular structure as well as concentration. In conclusion, whereas the baseline M~s~/M~o~ value in cartilage may be primarily due to the tissue collagen concentration, changes in M~s~/M~o~ may be due to physiological or pathophysiological changes in GAG concentration and tissue structure, and the measured M~s~/M~o~ may differentiate between various pathomimetic degradative procedures.