Magnetic Circular Dichroism and Absorption Spectra of Phosphinidene in Noble-Gas Matrices

Characterization of equilibrium intermed

Characterization of equilibrium intermediates in denaturant-induced unfolding of ferrous and ferric cytochromes c using magnetic circular dichroism, circular dichroism, and optical absorption spectroscopies

✍ Yiren G. Thomas; Robert A. Goldbeck; David S. Kliger 📂 Article 📅 2000 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 159 KB 👁 1 views

Protein unfolding during guanidine HCl denaturant titration of the reduced and oxidized forms of cytochrome c is monitored with magnetic circular dichroism (MCD), natural CD, and absorption of the heme bands and far-UV CD of the amide bands. Direct MCD spectral evidence is presented for bis-histidin