Magnesium fractions in serum of healthy individuals and capd patients, measured by an ion-selective electrode and ultrafiltration
✍ Scribed by Henk J. Huijgen; Huub E. Van Ingen; Wim TH. Kok; Gerard T.B. Sanders
- Publisher
- Elsevier Science
- Year
- 1996
- Tongue
- English
- Weight
- 553 KB
- Volume
- 29
- Category
- Article
- ISSN
- 0009-9120
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✦ Synopsis
Objectives:
Validation and comparison of a magnesium ion-selective electrode (ise) with a cation-exchange resin technique, followed by determination of all magnesium fractions in serum of healthy volunteers and continuous ambulatory peritoneal dialysis (capd) patients.
Design and methods:
The analytical aspect has been studied by measuring the influence of complexing agents on the fraction ionized magnesium (frimg2+). a theoretical approximation of frimg2+, based on mass equilibria and complexation constants, was calculated and compared with the measurements.
Results:
Ise measurements showed good agreement with theory. reference values of the ionized, protein-bound, and complexed magnesium fractions were (mean +/- sd) 0.65 +/- 0.04, 0.27 +/- 0.04, and 0.08 +/- 0.03, respectively. fractions obtained in the capd group were 0.62 +/- 0.04, 0.22 +/- 0.05, and 0.16 +/- 0.05, respectively, and differed significantly from the values of the reference population.
Conclusions:
All known serum magnesium parameters can be established by a combination of ultrafiltration, atomic absorption spectrometry, and ise measurements. unknown complexing compounds most probably account for the increased fraction of complexed magnesium in the serum of capd patients.