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Magnesium-DNA interactions from interpretation of 25Mg-nmr relaxation rates: Field and coion dependence

✍ Scribed by Laura A. Wright; Laura E. Lerner

Publisher: Wiley (John Wiley & Sons)
Year: 1994
Tongue: English
Weight: 826 KB
Volume: 34
Category: Article
ISSN: 0006-3525
DOI: 10.1002/bip.360340602

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✦ Synopsis

We have used *'Mg-nmr to investigate the binding of magnesium ions to double-stranded DNA. We have measured line shapes for "Mg in the presence of monodisperse calf thymus DNA ( 160 base pairs; b.p.) (magnesium : phosphate = 2.0) a t two different field strengths, 11.75 T and 7.05 T, and used the isotropic model of two-site exchange developed by Westlund and Wennerstrom to simultaneously fit the line shapes a t both field strengths. This model does not reproduce the observed field dependence. This is in contrast to a previous study

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