Macrophage Migration Inhibitory Factor: Methods and Protocols

✍ Scribed by James Harris, Eric F. Morand

• Publisher: Springer US;Humana
• Year: 2020
• Tongue: English
• Leaves: 268
• Series: Methods in Molecular Biology 2080
• Edition: 1st ed. 2020
• Category: Library

✦ Synopsis

This volume looks at--and discusses the techniques of--a range of areas in biology in which macrophage migration inhibitory factor (MIF) is studied. Topics covered in this book include methods to quantify MIF in cell supernatants and human samples, using ELISA and western blot; detection and analysis of intracellular MIF using flow cytometry; genotyping of human MIF polymorphisms, detection of MIF expression in a marine invertebrate using rapid amplification of cDNA RACE; studying the MIF receptor CD74 in tissues using immunohistochemistry; and studying MIF in models of disease such as asthma, Plasmodium infection, and gout. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and practical, Macrophage Migration Inhibitory Factor: Methods and Protocols is a valuable resource that will help researchers gain a new understanding of MIF biology in health and disease.

✦ Table of Contents

Front Matter ....Pages i-xi
Studying the Pro-Migratory Effects of MIF (Adrian Hoffmann, Leon Christian Zwißler, Omar El Bounkari, Jürgen Bernhagen)....Pages 1-18
In Vitro Approaches for Investigating the Influence of MIF on Leukocyte-Endothelial Cell Interactions Under Flow Conditions (Michael J. Hickey)....Pages 19-25
Using Intravital Microscopy to Study the Role of MIF in Leukocyte Trafficking In Vivo (M. Ursula Norman, Michael J. Hickey)....Pages 27-37
Analysis of Secreted MIF from Cultured Murine Bone Marrow-Derived Immune Cells (Tali Lang)....Pages 39-46
Measuring MIF in Biological Fluids (Fabien B. Vincent, Tali Lang)....Pages 47-56
Flow Cytometry Phenotyping of Bone Marrow-Derived Macrophages from Wild-Type and Mif−/− Mice (Jacqueline K. Flynn, Nadia S. Deen, James Harris)....Pages 57-66
Genotyping Two Promoter Polymorphisms in the MIF Gene: A −794 CATT5–8 Microsatellite Repeat and a −173 G/C SNP (Lin Leng, Edwin Siu, Richard Bucala)....Pages 67-84
Staining MIF in Cells for Confocal Microscopy (James Harris)....Pages 85-91
Microscopy Methods for Imaging MIF and Its Interaction Partners (Kirstin D. Elgass, Sarah J. Creed, Ina Rudloff)....Pages 93-114
Co-Immunoprecipitation of Macrophage Migration Inhibitory Factor (Jawad H. Abidi, James Harris, Nadia S. Deen)....Pages 115-122
Concurrent Immunohistochemical Localization and Western Blot Analysis of the MIF Receptor, CD74, in Formalin-Fixed, Paraffin-Embedded Tissue (Amanda Graham, Warren B. Nothnick)....Pages 123-134
Methods to Determine the Effects of MIF on In Vitro Osteoclastogenesis Using Murine Bone Marrow-Derived Cells and Human Peripheral Blood Mononuclear Cells (Ran Gu)....Pages 135-145
Inducing and Inhibiting Autophagy to Investigate Its Interactions with MIF (Nadia S. Deen, Jacinta P. Lee, James Harris)....Pages 147-158
Assays for Measuring the Role of MIF in NLRP3 Inflammasome Activation (Anita A. Pinar, James Harris)....Pages 159-172
Assays for Inducing and Measuring Cell Death to Detect Macrophage Migration Inhibitory Factor (MIF) Release (Shahrzad Zamani, Eric F. Morand, Jacqueline K. Flynn)....Pages 173-183
Assessing the Role of MIF in Plasmodium spp. Infections Using Ex Vivo Models (Elizabeth H. Aitken)....Pages 185-192
The Effect of Macrophage Migration Inhibitory Factor on Intestinal Permeability: FITC-Dextran Serum Measurement and Transmission Electron Microscopy (Milica Vujičić, Sanja Despotović, Tamara Saksida, Ivana Stojanović)....Pages 193-201
Investigating MIF in Mouse Models of Severe Corticosteroid-Resistant Neutrophilic Asthma (Venkata Sita Rama Raju Allam, Maria B. Sukkar)....Pages 203-212
Investigating MIF in Mouse Model of Gout (Izabela Galvão, Allysson Cramer, Flavio Almeida Amaral)....Pages 213-222
5′ and 3′ RACE Method to Obtain Full-Length 5′ and 3′ Ends of Ciona robusta Macrophage Migration Inhibitory Factors Mif1 and Mif2 cDNA (Aiti Vizzini)....Pages 223-235
Purification of Antibodies Against Entamoeba histolytica MIF and Their Use in Analyzing Human and Mouse Samples (Laura Farr, Koji Watanabe, Shannon Moonah)....Pages 237-247
Studying Plant MIF/D-DT-Like Genes and Proteins (MDLs) (Dzmitry Sinitski, Katrin Gruner, Jürgen Bernhagen, Ralph Panstruga)....Pages 249-261
Back Matter ....Pages 263-265

✦ Subjects

Biomedicine; Immunology; Cell Biology; Molecular Medicine