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Macrophage-like cells from explant cultures of rat sciatic nerve produce apolipoprotein E

✍ Scribed by Dr. J. F. Goodrum; D. L. Novicki

Publisher: John Wiley and Sons
Year: 1988
Tongue: English
Weight: 671 KB
Volume: 20
Category: Article
ISSN: 0360-4012
DOI: 10.1002/jnr.490200408

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✦ Synopsis

Apolipoprotein E is synthesized and secreted by degenerating peripheral nerve, but the role of resident endoneurial cells in this process is not clear. To exclude the involvement of nonresident cells, we examined the cellular source of endoneurial apolipoprotein E in explant cultures of rat sciatic nerve. The cellular outgrowth from these explant cultures released apolipoprotein E into the culture medium. The cellular outgrowth contained fibroblasts, Schwann cells, and a population of cells with many phenotypic characteristics of macrophages, including the production of apolipoprotein E. No other cell type in the cultures appeared to contribute to this production. These data suggest that apolipoprotein E is produced by resident endoneurial cells in explant cultures and that these cells are macrophages.

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