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Macromolecular synthesis and stability in growth-arrested BALB/C-3T3 cells

✍ Scribed by Walker Wharton; W. J. Pledger

Publisher
John Wiley and Sons
Year
1982
Tongue
English
Weight
529 KB
Volume
19
Category
Article
ISSN
0730-2312

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✦ Synopsis

Abstract

Concentrations of methylglyoxal bis‐(guanylhydrazone) (mGBG) that inhibited serum‐stimulated BALB/c‐3T3 cells in late G~1~ caused a marked inhibition of ^3^H‐leucine incorporation during a 20‐min incubation. No decrease was observed in the incorporation of ^3^H‐uridine during a 20‐min incubation; however, the amount of acid‐insoluble ^3^H‐uridine in mGBG‐treated cultures was decreased when the incubation period was longer than 20 min. The amount of the decrease in the accumulation of incorporated ^3^H‐uridine was directly proportional to the length of the incorporation time. Between 10 and 12 h after quiescent BALB/C‐3T3 cells were serum‐stimulated in mGBG no additional ^3^H‐uridine was accumulated. The stability of the incorporated ^3^H‐uridine, as determined by acid‐insoluble radioactivity remaining after the addition of actinomycin D, was less in cells cultured in mGBG. Exogenous spermine or spermidine reversed the inhibition of ^3^H‐uridine accumulation in acid‐insoluble material produced by mGBG as well as the decrease in stability of the incorporated ^3^H‐uridine in acid‐insoluble material. The effects of mGBG on both the incorporation of ^3^H‐uridine and the stability of the incorporated ^3^H‐uridine can apparently be accounted for by an effect on ribosomal RNA.

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